Hexitol metabolism in Escherichia coli.

نویسندگان

J B WOLFF

N O KAPLAN

چکیده

D-Sorbitol and dulcitol (galactitol), which, together with D-mannitol, are the three naturally occurring hexitols, have been known since the last quarter of the nineteenth century. Many fungi can utilize sorbitol for growth, but dulcitol is assimilated poorly by Aspergillus niger and a few yeasts. Among bacterial fermentations of these hexitols the best known are those by members of the Acetobacter group. These species will readily oxidize mannitol to fructose, sorbitol to sorbose, glycerol to dihydroxyacetone, and erythritol to L-erythrulose, but most of the organisms will not attack dulcitol. In a study of oxidation by Acetobacter suboxydans, King and Cheldelin (1952) found that the initial step of sorbitol oxidation (1 atom of oxygen) in resting cells was not affected by 4 X 10-4 M 2,4dinitrophenol, and thus they concluded that this step to sorbose was independent of the esterification of inorganic phosphate. Closely related to Acetobacter organisms are the pseudomonads studied by Sebek and Randles (1952). Only one strain of Pseudomonas fltorescens grew on sorbitol; none of the seven strains showed growth on dulcitol. As growth on the hexitols was adaptive, the nature of their oxidation products could be studied by the technique of "simultaneous adaptation" as well as by actual isolation. The former indicated fructose to be the product of sorbitol oxidation, while

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